High-Throughput Analysis of Subcellular Localization in Budding Yeast

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Air date:	Friday, February 6, 2004, 1:00:00 PM Time displayed is Eastern Time, Washington DC Local
Views:	Total views: 100 * This only includes stats from October 2011 and forward.
Category:	Proteomics
Runtime:	01:15:11
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Description:	A fundamental goal of cell biology is to define the functions of proteins in the context of compartments that organize them in the cellular milieu. We have constructed and analyzed a collection of yeast strains expressing full-length, chromosomally-tagged green fluorescent protein (GFP) fusion proteins. We classified these proteins, representing 75% of the yeast proteome, into twenty-two distinct subcellular localization categories and provide localization information for 70% of previously unlocalized proteins. Analysis of this high-resolution, high-coverage localization dataset in the context of transcriptional and protein-protein interaction data helps reveal the logic of transcriptional co-regulation and provides a comprehensive view of interactions within and between organelles in eukaryotic cells. For more information, visit http://proteome.nih.gov

NLM Title:	High-throughput analysis of subcellular localization in budding yeast / Erin O'Shea.
Author:	O'Shea, Erin. National Institutes of Health (U.S.)
Publisher:
Abstract:	(CIT): A fundamental goal of cell biology is to define the functions of proteins in the context of compartments that organize them in the cellular milieu. We have constructed and analyzed a collection of yeast strains expressing full-length, chromosomally-tagged green fluorescent protein (GFP) fusion proteins. We classified these proteins, representing 75% of the yeast proteome, into twenty-two distinct subcellular localization categories and provide localization information for 70% of previously unlocalized proteins. Analysis of this high-resolution, high-coverage localization dataset in the context of transcriptional and protein-protein interaction data helps reveal the logic of transcriptional co-regulation and provides a comprehensive view of interactions within and between organelles in eukaryotic cells. For more information, visit the Proteomics Interest Group.
Subjects:	Gene Expression Regulation Protein Transport Proteomics Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae
Publication Types:	Lecture Webcast
Rights:	This is a work of the United States Government. No copyright exists on this material. It may be disseminated freely.
NLM Classification:	QU 58.5
NLM ID:	101268357
CIT Live ID:	3063
Permanent link:	https://videocast.nih.gov/watch=3063

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