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Genome Maintenance Up Close and Personal: Eavesdropping on Single Molecular Conversations

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Air date: Wednesday, April 9, 2014, 3:00:00 PM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 353, (92 Live, 261 On-demand)
Category: WALS - Wednesday Afternoon Lectures
Runtime: 00:57:26
Description: Single-molecule detection has opened vast avenues to investigate aspects of biological systems that are inaccessible by any other technique. Research in the Ha lab is focused on pushing the limits of single-molecule detection methods to study biological systems with multiple components to better mimic the cellular conditions. In the first part of the lecture, Dr. Ha will describe the surprising and deep insights revealed by multi-color fluorescence resonance energy transfer (FRET) and fluorescence-force spectroscopy on the dynamics of DNA repair proteins on single-stranded DNA. In the second part of the lecture, he will describe a single-molecule pull-down (SiMPull) assay that combines the principles of a conventional pull-down assay with single-molecule fluorescence microscopy and enables direct visualization of individual cellular protein complexes. SiMPull can reveal how many proteins and which kinds are present in the in vivo complex. SiMPull is also widely applicable to various signalling proteins found in the cytosol, membrane and cellular organelles, and to endogenous protein complexes from animal tissue extracts.

For more information go to http://wals.od.nih.gov/
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NLM Title: Genome maintenance up close and personal : eavesdropping on single molecular conversations / Taekjip Ha.
Author: Ha, Taekjip.
National Institutes of Health (U.S.),
Publisher:
Abstract: (CIT): Single-molecule detection has opened vast avenues to investigate aspects of biological systems that are inaccessible by any other technique. Research in the Ha lab is focused on pushing the limits of single-molecule detection methods to study biological systems with multiple components to better mimic the cellular conditions. In the first part of the lecture, Dr. Ha will describe the surprising and deep insights revealed by multi-color fluorescence resonance energy transfer (FRET) and fluorescence-force spectroscopy on the dynamics of DNA repair proteins on single-stranded DNA. In the second part of the lecture, he will describe a single-molecule pull-down (SiMPull) assay that combines the principles of a conventional pull-down assay with single-molecule fluorescence microscopy and enables direct visualization of individual cellular protein complexes. SiMPull can reveal how many proteins and which kinds are present in the in vivo complex. SiMPull is also widely applicable to various signalling proteins found in the cytosol, membrane and cellular organelles, and to endogenous protein complexes from animal tissue extracts.
Subjects: DNA Repair
DNA, Single-Stranded--chemistry
Fluorescence Resonance Energy Transfer
Microscopy, Fluorescence
Multiprotein Complexes--chemistry
Publication Types: Lecture
Webcast
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Caption Text: Download Caption File
NLM Classification: QU 475
NLM ID: 101631789
CIT Live ID: 14054
Permanent link: https://videocast.nih.gov/watch=14054