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Modern microscopy has developed from a convergence of advances in cell manipulation, probe chemistry, solid state physics, electronics, optics, and image analysis. This meeting brings together 36 leading scientists from these diverse fields to review recent progress, chart new directions, and promote interactions. In addition to acquainting practicing microscopists with the newest developments, this meeting is also designed to introduce investigators in chemistry, biochemistry, biophysics, and the physical sciences to opportunities for research and development in this expanding interdisciplinary field. The meeting will complement recent and forthcoming initiatives from the NIH that seek to promote imaging technology development and interdisciplinary research.