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Biowulf Seminar Series: Cryo-EM studies of glutamate receptors and nucleosomes

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Air date: Wednesday, May 9, 2018, 11:00:00 AM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 71, (25 Live, 46 On-demand)
Category: Special
Runtime: 00:56:39
Description: Biowulf Seminar Series

Electron microscopy (EM) has been traditionally used to calculate three-dimensional reconstructions that were interpreted using high-resolution structures determined by X-ray crystallography and NMR to provide biological insights into the function of large macromolecular assemblies. However, recent technological and methodological developments in cryo-EM have now made it possible to routinely study biological macromolecules of lower molecular weight at near-atomic resolutions, thus opening up an interesting area of determining three dimensional structures of novel complexes. Here, I will discuss the following two examples to highlight the role of single particle cryo-EM in advancing the structural biology: 1. Ionotropic glutamate receptors (iGluRs) – here we used single particle cryo-EM in conjunction with high resolution structures determined by X-ray crystallography to reveal key conformational changes involved in receptor gating [references 1 and 2]. 2. Structure of the centromeric nucleosome in complex with the kinetochore protein CENP-N – to illustrate an example where we utilized the cryo-EM density map to carry out ab initio modeling of the CENP-N component without any prior knowledge of its three-dimensional structure [reference 3]. With these and ongoing advances in cryo-EM, it is now feasible to study other previously intractable biological complexes

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Author: Sagar Chittori, Ph.D., NCI, NIH
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CIT Live ID: 27654
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