NIH VideoCast - Antibodies, Genome Stability, and Cancer

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Antibodies, Genome Stability, and Cancer

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Air date:	Wednesday, March 27, 2013, 3:00:00 PM Time displayed is Eastern Time, Washington DC Local
Views:	Total views: 460 (108 Live, 352 On-demand)
Category:	WALS - Wednesday Afternoon Lectures
Runtime:	01:10:05
Closed captioning:	Check box to display CC outside of the video Note: You can drag the captioning window around and resize it
Description:	Wednesday Afternoon Lecture Series Dr. Alt and his group developed high-throughput, genome-wide translocation sequencing (HTGTS) to elucidate mechanisms that generate and join DNA double strand breaks (DSBs) and mediate translocations. He will discuss several new studies that employ the HTGTS approach and other approaches to address questions about the immunoglobulin heavy locus (IgH) class switch recombination (CSR) including how CSR DSBs are joined in a productive (deletional versus inversional) orientation in the chromosome; how IgH DSBs are synapsed for joining over several 100-kilobyte chromosomal distances to effect physiological levels of CSR; and the potential roles of the nuclear protein kinase ataxia telangiectasia mutated (ATM)-dependent DNA DSB response factors, including 53BP1, in the CSR process. He will also discuss the use of HTGTS to elucidate novel mechanisms that lead to V(D)J recombination-associated oncogenic chromosomal translocations and oncogene activation in ATM-deficient B- and T-cell lymphomas.

NLM Title:	Antibodies, genome stability, and cancer / Fred Alt.
Author:	Alt, Frederick W. National Institutes of Health (U.S.),
Publisher:
Abstract:	(CIT): Wednesday Afternoon Lecture Series Dr. Alt and his group developed high-throughput, genome-wide translocation sequencing (HTGTS) to elucidate mechanisms that generate and join DNA double strand breaks (DSBs) and mediate translocations. He will discuss several new studies that employ the HTGTS approach and other approaches to address questions about the immunoglobulin heavy locus (IgH) class switch recombination (CSR) including how CSR DSBs are joined in a productive (deletional versus inversional) orientation in the chromosome; how IgH DSBs are synapsed for joining over several 100-kilobyte chromosomal distances to effect physiological levels of CSR; and the potential roles of the nuclear protein kinase ataxia telangiectasia mutated (ATM)-dependent DNA DSB response factors, including 53BP1, in the CSR process. He will also discuss the use of HTGTS to elucidate novel mechanisms that lead to V(D)J recombination-associated oncogenic chromosomal translocations and oncogene activation in ATM-deficient B- and T-cell lymphomas.
Subjects:	DNA Breaks, Double-Stranded High-Throughput Nucleotide Sequencing Immunoglobulin Class Switching Sequence Analysis, DNA Translocation, Genetic--immunology
Publication Types:	Lecture Webcast

NLM Classification:	QU 477
NLM ID:	101607218
CIT Live ID:	12314
Permanent link:	https://videocast.nih.gov/watch=12314