NIH VideoCast - IIG Seminar: Defining malaria vaccine responses by single B cell IG sequencing and plasma IgG proteomics

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IIG Seminar: Defining malaria vaccine responses by single B cell IG sequencing and plasma IgG proteomics

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422 Views

Air date:	Wednesday, June 17, 2020, 2:15:00 PM Time displayed is Eastern Time, Washington DC Local
Views:	Total views: 422 (181 Live, 241 On-demand)
Category:	Immunology
Runtime:	00:44:50
Closed captioning:	Check box to display CC outside of the video Note: You can drag the captioning window around and resize it
Description:	IIG Seminar Malaria elimination is a global priority and WHO has projected that malaria deaths could double due to COVID19-related health care disruptions. Vaccines have been pivotal for campaigns to eliminate or eradicate other infectious diseases. Malaria transmission blocking vaccines (TBVs) target surface antigens expressed by parasites during their development in mosquitoes in order to interrupt transmission and contribute to malaria elimination. We collected antigen-specific memory B cells from Malian adults immunized with TBV and obtained B cell receptor IG sequences that were used to define the antibody repertoire and to generate the first recombinant human mAbs against Plasmodium gamete surface antigen. One potent humAb binds to a broad and conserved epitope on Plasmodium gametes, suggesting a path to improve TBV design. In addition, we studied plasma antibody repertoire using proteomics of antigen-specific F(ab’)2 from vaccinees and identified antibody peptides that provide insights into V gene usage. Our data support further development of TBV to pursue malaria elimination and provide insights into key TBV epitopes that will inform the design of improved malaria vaccines. For more information go to https://www.niaid.nih.gov/research/immunology-interest-group

NLM Title:	Defining malaria vaccine responses by single B cell Ig sequencing and plasma IgG proteomics / Camila Coelho.
Author:	Coelho, Camila. National Institutes of Health (U.S.). Immunology Interest Group,
Publisher:
Abstract:	(CIT): IIG Seminar Malaria elimination is a global priority and WHO has projected that malaria deaths could double due to COVID19-related health care disruptions. Vaccines have been pivotal for campaigns to eliminate or eradicate other infectious diseases. Malaria transmission blocking vaccines (TBVs) target surface antigens expressed by parasites during their development in mosquitoes in order to interrupt transmission and contribute to malaria elimination. We collected antigen-specific memory B cells from Malian adults immunized with TBV and obtained B cell receptor IG sequences that were used to define the antibody repertoire and to generate the first recombinant human mAbs against Plasmodium gamete surface antigen. One potent humAb binds to a broad and conserved epitope on Plasmodium gametes, suggesting a path to improve TBV design. In addition, we studied plasma antibody repertoire using proteomics of antigen-specific F(ab")2 from vaccinees and identified antibody peptides that provide insights into V gene usage. Our data support further development of TBV to pursue malaria elimination and provide insights into key TBV epitopes that will inform the design of improved malaria vaccines.
Subjects:	B-Lymphocytes--immunology Immunoglobulin G--physiology Malaria Vaccines--immunology Malaria--prevention & control
Publication Types:	Lecture Webcast

NLM Classification:	WC 765
NLM ID:	101769633
CIT Live ID:	37965
Permanent link:	https://videocast.nih.gov/watch=37965

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