Skip Navigation

Application of MALDI-Ion Mobility Mass Spectrometry in Biological Analysis

Loading video...

126 Views

Air date: Thursday, February 1, 2018, 9:30:00 AM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 126 (8 Live, 118 On-demand)
Category: Proteomics
Runtime: 00:48:23
Description: Proteomics Interest Group

Ion mobility (IM) spectrometry is a robust method that allows for the rapid separation and detection of a wide range of compounds. In this technique, ions in the drift cell obtain an average drift velocity from an electric field based upon their collision cross section (W) or shape, which allows for the separation of molecules depending upon their three-dimensional structure. Combining matrix-assisted laser desorption/ionization (MALDI) mass spectrometry with IM results in the fast sorting of biomolecules in complex mixtures along trend lines. In this 2D analysis of biological families, lipids, peptides, and nucleotides are separated from each other by differences in their ion mobility drift times in a timescale of hundreds of microseconds. Molecular ions of similar chemical type fall along trend lines when plotted in two-dimensional plots of ion mobility drift time as a function of m/z. We have analyzed a wide variety of samples using MALDI-IM MS. This talk will highlight the use of MALDI-IM MS for the analysis of lipids and it advantages in mass spectrometry imaging.
Author: Shelley Jackson, Ph.D., Staff Scientist, NIDA, NIH
 
CIT Live ID: 27093
Permanent link: https://videocast.nih.gov/watch=27093

Related Videos...

Click Here to Play Video.
08:36:04
NIH Consensus Development Conference on Diagnosis and Management of Dental Caries Throughout Life (Day 1)
[Conferences]
3/26/2001
Click Here to Play Video.
06:18:49
The Next Step: Exploring the Proteome
[Conferences]
5/21/2001
Click Here to Play Video.
04:24:45
Alliance for Cellular Signaling
[Conferences]
5/25/2001
Click Here to Play Video.
08:53:31
Standards in Proteomics: Building Blocks, Biological Pathways and Networks (Day 1)
[Conferences]
1/4/2005
Click Here to Play Video.
08:02:51
Metabolomics Standards Workshop (Day 1)
[Conferences]
8/1/2005
Click Here to Play Video.
07:18:19
State of Metabolomics Technologies in Translational Research
[Conferences]
9/17/2010
Click Here to Play Video.
07:18:32
RAS Initiative Symposium (Day 2)
[NIH Only]
12/16/2015
Click Here to Play Video.
05:49:01
RAS Second Symposium (Day 1)
[NIH Only]
12/6/2017
Click Here to Play Video.
06:51:47
Fourth Geroscience Summit: Geroscience for the Next Generation (Day 2)
[Conferences]
4/25/2023
Click Here to Play Video.
07:03:04
The Evolution of Mental Health Research
[Conferences]
9/13/2023