NIH VideoCast - Detection of Dichlorvos Adducts on Proteins from a Liver-derived Cell Line

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Detection of Dichlorvos Adducts on Proteins from a Liver-derived Cell Line

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Air date:	Thursday, March 6, 2014, 10:00:00 AM Time displayed is Eastern Time, Washington DC Local
Views:	Total views: 132 (4 Live, 128 On-demand)
Category:	Proteomics
Runtime:	00:49:54
Closed captioning:	Check box to display CC outside of the video Note: You can drag the captioning window around and resize it
Description:	Proteomics Interest Group The toxicity of dichlorvos (DDVP), an organophosphate (OP) pesticide, classically results from modification of the serine in the active sites of cholinesterases. However, DDVP also forms adducts on unrelated targets such as transferrin and albumin, suggesting that DDVP could cause perturbations in cellular processes by modifying non-cholinesterase targets. Here, we identify novel DDVP-modified targets in lysed human hepatocyte-like cells (HepaRG) using a direct liquid chromatography-mass spectrometry (LC-MS) assay of cell lysates incubated with DDVP or using a competitive pull-down experiments with a biotin-linked organophosphorus compound (10-fluoroethoxyphosphinyl-N-biotinamidopentyldecanamide; FP-biotin), which competes with DDVP for similar binding sites. We show that DDVP forms adducts to several proteins important for the cellular metabolic pathways and differentiation, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin. We validated the results using purified proteins and enzymatic assays. The study not only identified novel DDVP-modified targets but also suggested that the modification directly inhibits the enzymes. The current approach provides information for future hypothesis-based studies to understand the underlying mechanism of toxicity of DDVP in non-neuronal tissues. For more information go to http://proteome.nih.gov

NLM Title:	Detection of dichlorvos adducts on proteins from a liver-derived cell line / John A. Lewis ; Proteomics Interest Group.
Author:	Lewis, John A. National Institutes of Health (U.S.). Proteomics Interest Group,
Publisher:
Abstract:	(CIT): Proteomics Interest Group The toxicity of dichlorvos (DDVP), an organophosphate (OP) pesticide, classically results from modification of the serine in the active sites of cholinesterases. However, DDVP also forms adducts on unrelated targets such as transferrin and albumin, suggesting that DDVP could cause perturbations in cellular processes by modifying non-cholinesterase targets. Here, we identify novel DDVP-modified targets in lysed human hepatocyte-like cells (HepaRG) using a direct liquid chromatography-mass spectrometry (LC-MS) assay of cell lysates incubated with DDVP or using a competitive pull-down experiments with a biotin-linked organophosphorus compound (10-fluoroethoxyphosphinyl-N-biotinamidopentyldecanamide; FP-biotin), which competes with DDVP for similar binding sites. We show that DDVP forms adducts to several proteins important for the cellular metabolic pathways and differentiation, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin. We validated the results using purified proteins and enzymatic assays. The study not only identified novel DDVP-modified targets but also suggested that the modification directly inhibits the enzymes. The current approach provides information for future hypothesis-based studies to understand the underlying mechanism of toxicity of DDVP in non-neuronal tissues.
Subjects:	DNA Adducts Dichlorvos--toxicity Hepatocytes--drug effects Insecticides--toxicity
Publication Types:	Lecture Webcast

NLM Classification:	WA 240
NLM ID:	101629954
CIT Live ID:	13790
Permanent link:	https://videocast.nih.gov/watch=13790

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