The highly selective exchange of proteins and RNA between the nucleus and cytoplasm is essential for cell function, and precisely regulated during cell division, differentiation and death. Many aspects of this process are poorly understood and have direct impacts on human disease.
This lecture will have three objectives.
One, it will provide background on current models for messenger RNA export from the nucleus to the cytoplasm, and points for key cellular regulation of gene expression.
Second, the lecture documents the discovery of essential factors for coupling mRNA export and translation in the budding yeast and cell culture models.
Finally, an overall goal is to reveal pathophysiological impacts on development from the study of mRNA export factors in the zebrafish model and human disease linkage analysis.
This work provides a direct illustration of how discoveries from the analysis of single cell machineries are required for understanding multicellular organism development and pathophysiology.