Skip Navigation


CIT can broadcast your seminar, conference or meeting live to a world-wide audience over the Internet as a real-time streaming video. The event can be recorded and made available for viewers to watch at their convenience as an on-demand video or a downloadable file. CIT can also broadcast NIH-only or HHS-only content.

Emerging Fluorescence Technologies for Analysis of Protein Localization and Organelle Dynamics

Loading video...

 
   
Air date: Wednesday, April 4, 2007, 3:00:00 PM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 130 * This only includes stats from October 2011 and forward.
Category: WALS - Wednesday Afternoon Lectures
Runtime: 01:08:00
Description: The development of fluorescent proteins as molecular tags over the past decade has splurred a revolution by allowing complex biochemical processes to be correlated with the functioning of proteins in livings cells. Fluorescent proteins such as green fluorescent protein (GFP) from the jelly fish Aequorea Victoria and its variants can be fused to virtually any protein of interest to analyze protein geography, movement and chemistry in living cells. As such, they have provided an important new tool for understanding protein function, filling an urgent need now that the genome sequence of many organisms is complete. Among the new fluorescent proteins are those that can be photoactivated. These molecules are invisible until activated by a specific wavelength of light, at which point they become brightly fluorescent. I will discuss results from two new techniques employing photoactivatable fluorescent proteins: in cellula pulse-chase analysis and photoactivated localization microscopy (PALM).
Debug: Show Debug
NLM Title: Emerging fluorescence technologies for analysis of protein localization and organelle dynamics / Jennifer Lippincott-Schwartz.
Author: Lippincott-Schwartz, Jennifer.
National Institutes of Health (U.S.)
Publisher:
Abstract: (CIT): The development of fluorescent proteins as molecular tags over the past decade has splurred a revolution by allowing complex biochemical processes to be correlated with the functioning of proteins in livings cells. Fluorescent proteins such as green fluorescent protein (GFP) from the jelly fish Aequorea Victoria and its variants can be fused to virtually any protein of interest to analyze protein geography, movement and chemistry in living cells. As such, they have provided an important new tool for understanding protein function, filling an urgent need now that the genome sequence of many organisms is complete. Among the new fluorescent proteins are those that can be photoactivated. These molecules are invisible until activated by a specific wavelength of light, at which point they become brightly fluorescent. I will discuss results from two new techniques employing photoactivatable fluorescent proteins: in cellula pulse-chase analysis and photoactivated localization microscopy (PALM).
Subjects: Luminescent Proteins
Microscopy, Fluorescence--methods
Organelles
Proteins--analysis
Proteins--metabolism
Publication Types: Lecture
Webcasts
Download: To download this event, select one of the available bitrates:
[384k]    How to download a Videocast
NLM Classification: QU 55
NLM ID: 101306056
CIT Live ID: 5195
Permanent link: https://videocast.nih.gov/launch.asp?13738