CIT can broadcast your seminar, conference or meeting live to a world-wide
audience over the Internet as a real-time streaming video. The event can
be recorded and made available for viewers to watch at their convenience
as an on-demand video or a downloadable podcast. CIT can also broadcast
NIH-only or HHS-only content.
In eukaryotic cells, most membrane proteins are integrated into the membrane of the endoplasmic reticulum (ER) co-translationally at sites termed translocons.
Each translocon forms an aqueous pore that spans the bilayer, yet the permeability barrier of the ER membrane is maintained during secretory protein translocation or membrane protein integration at the translocon. Small ion movement through the translocon pore during integration is regulated by a complex choreography of interactions between the ribosome, translocon, BiP, and probably other molecular species. We have used fluorescence spectroscopy, fluorescence resonance energy transfer, and photocrosslinking to examine intermediates at various stages of integration.