Skip Navigation


CIT can broadcast your seminar, conference or meeting live to a world-wide audience over the Internet as a real-time streaming video. The event can be recorded and made available for viewers to watch at their convenience as an on-demand video or a downloadable podcast. CIT can also broadcast NIH-only or HHS-only content.

Testing the Significance of Protein Identification Results

Loading video...

 
   
Air date: Friday, December 5, 2003, 10:00:00 AM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 18 * This only includes stats from October 2011 and forward.
Category: Proteomics
Runtime: 00:50:33
Description: Mass spectrometry and database searching is widely used for protein identification. The proteins in the sample are first separated to obtain a mixture of the proteins of interest. These protein mixtures are then digested with a proteolytic enzyme. The masses of the resulting peptides and their fragments are measured and compared with calculated peptide masses from a protein sequence collection. A score is calculated for the comparison and the protein sequences in the collection are ranked according to the calculated score. Different search engines calculate the score in different ways. The meaning of these scores is in general not easily understood by the non-expert user and they are not amenable to automation.

Proteome Interest Group
Debug: Show Debug
NLM Title: Testing the significance of protein identification results [electronic resource] / David Fenyo.
Author: Fenyo, David.
National Institutes of Health (U.S.)
Publisher:
Abstract: (CIT): Mass spectrometry and database searching is widely used for protein identification. The proteins in the sample are first separated to obtain a mixture of the proteins of interest. These protein mixtures are then digested with a proteolytic enzyme. The masses of the resulting peptides and their fragments are measured and compared with calculated peptide masses from a protein sequence collection. A score is calculated for the comparison and the protein sequences in the collection are ranked according to the calculated score. Different search engines calculate the score in different ways. The meaning of these scores is in general not easily understood by the non-expert user and they are not amenable to automation. Proteome Interest Group.
Subjects: Algorithms
Computer Simulation
Proteins--analysis
Research Design
Publication Types: Lectures
Webcasts
Rights: This is a work of the United States Government. No copyright exists on this material. It may be disseminated freely.
Download: To download this event, select one of the available bitrates:
[384k]    How to download a Videocast
NLM Classification: QU 55
NLM ID: 101268307
CIT Live ID: 2956
Permanent link: https://videocast.nih.gov/launch.asp?11701