Skip Navigation


CIT can broadcast your seminar, conference or meeting live to a world-wide audience over the Internet as a real-time streaming video. The event can be recorded and made available for viewers to watch at their convenience as an on-demand video or a downloadable file. CIT can also broadcast NIH-only or HHS-only content.

High-throughput quantitative proteomics

Loading video...

133 Views  
   
Air date: Thursday, May 3, 2018, 9:30:00 AM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 133, (19 Live, 114 On-demand)
Category: Proteomics
Runtime: 01:09:56
Description: ProtIG Lecture Series

The ability to rapidly identify functional proteins with post translation modifications (PTMs) is a crucial element in understanding cellular function. Protein PTMs have emerged in the post-genomic era critical features in regulating and diversifying protein biological activity, but identifying specific proteoforms with PTMs and understanding their function on individual proteins is currently limited by the lack of effective and accessible analytical methods. Therefore, there is a crucial need to develop a high-throughput approach to functionally characterize intact proteins and their modified proteoforms at the systems level. The overall purpose of our research program is to develop and apply new top-down and functional mass spectrometry (MS) proteomics technologies to characterize intact proteins and their modified proteoforms. This talk includes our recently developed high-throughput quantitative top-down MS platform that couples an online 2D pH RP/RPLC separation and multiplexed TMT top-down quantitation to identify and quantify low abundance intact proteins and their modified proteoforms in complex biological samples. I will also discuss our results on detecting intact autoantibody biomarkers in systemic lupus erythematosus (SLE) patient serum samples.
Debug: Show Debug
Author: Si Wu, Ph.D., Assistant Professor, University of Oklahoma
Download: To download this event, select one of the available bitrates:
[64k]  [150k]  [240k]  [440k]  [740k]  [1040k]  [1240k]  [1440k]  [1840k]    How to download a Videocast
Caption Text: Download Caption File
CIT Live ID: 27648
Permanent link: https://videocast.nih.gov/launch.asp?23859