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From microbial immunity to genome editing

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Air date: Wednesday, June 28, 2017, 3:00:00 PM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 810, (472 Live, 338 On-demand)
Category: WALS - Wednesday Afternoon Lectures
Runtime: 01:06:44
Description: NIH Director's Wednesday Afternoon Lecture Series

The microbial CRISPR-Cas adaptive immune systems provide archaea and bacteria with a programmable defense against invading nucleic acids. Several years ago, Dr. Zhang and others reported that the effector module from Streptococcus pyogenes CRISPR-Cas9 (SpCas9) could be harnessed for genome editing. Following this work, Zhang's lab has discovered, characterized, and engineered additional Cas enyzmes for use as molecular biology tools. These include a novel class of CRISPR-Cas systems that use RNA-guided RNases, such as Cas13a (C2c2) and Cas13b. Leveraging the natural properties of Cas13a, the lab developed a single-molecule nucleic acid sensing platform termed SHERLOCK, which can be used for rapid pathogen detection and genotyping. Zhang is continuing to explore microbial diversity to identify novel enzymes and systems that could serve as the basis for the development of additional molecular biology tools and applying these tools to tackle complex biological questions..

For more information go to https://oir.nih.gov/wals
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NLM Title: From microbial immunity to genome editing / Feng Zhang.
Author: Zhang, Feng.
National Institutes of Health (U.S.),
Publisher:
Abstract: (CIT): NIH Director's Wednesday Afternoon Lecture Series The microbial CRISPR-Cas adaptive immune systems provide archaea and bacteria with a programmable defense against invading nucleic acids. Several years ago, Dr. Zhang and others reported that the effector module from Streptococcus pyogenes CRISPR-Cas9 (SpCas9) could be harnessed for genome editing. Following this work, Zhang's lab has discovered, characterized, and engineered additional Cas enyzmes for use as molecular biology tools. These include a novel class of CRISPR-Cas systems that use RNA-guided RNases, such as Cas13a (C2c2) and Cas13b. Leveraging the natural properties of Cas13a, the lab developed a single-molecule nucleic acid sensing platform termed SHERLOCK, which can be used for rapid pathogen detection and genotyping. Zhang is continuing to explore microbial diversity to identify novel enzymes and systems that could serve as the basis for the development of additional molecular biology tools and applying these tools to tackle complex biological questions.
Subjects: Clustered Regularly Interspaced Short Palindromic Repeats--immunology
DNA Breaks, Double-Stranded
DNA-Binding Proteins
Gene Editing
Publication Types: Lectures
Webcasts
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Caption Text: Download Caption File
NLM Classification: QU 470
NLM ID: 101710188
CIT Live ID: 23704
Permanent link: https://videocast.nih.gov/launch.asp?23381