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Pushing the envelope in biological microscopy: High speed imaging at and beyond the diffraction limit

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Air date: Friday, May 19, 2017, 12:00:00 PM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 134, (83 Live, 51 On-demand)
Category: NIH Director's Seminars
Runtime: 00:51:59
Description: Director's Seminar Series

Dr. Hari Shroff will discuss his lab's, the Section of High Resolution Optical Imaging (HROS), efforts to improve structured illumination microscopy (SIM) and light-sheet microscopy. SIM doubles the spatial resolution of light microscopy, requiring lower light intensities and acquisition times than other super-resolution techniques. Dr. Shroff will present SIM implementations that enable resolution doubling in live samples > 10-20x thicker than possible with conventional SIM, as well as hardware modifications that enable effectively ‘instant’ SIM imaging at rates 10-100x faster than other SIM. New applications of instant SIM, including combination with total internal reflection (TIRF) and with adaptive optics will also be discussed.

The second half of the talk will focus on the development of inverted selective plane illumination microscopy (iSPIM), and subsequent application to the noninvasive study of neurodevelopment in nematodes. Dr. Shroff will discuss progress that quadruples the axial resolution of iSPIM by using a second specimen view, enabling imaging with isotropic spatial resolution (dual-view iSPIM, or diSPIM). Newer multiview microscopes with more objectives and more views, further improving spatial resolution, will also be described. Applications of these technologies will be presented, including computational methods for untwisting worm embryos and calcium imaging in freely moving embryos. Finally, Dr. Shroff will discuss the current status of the new trans-NIH, advanced imaging and microscopy (AIM) facility which allows intramural researchers to use cutting edge optical imaging systems developed at NIH.
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NLM Title: Pushing the envelope in biological microscopy : high speed imaging at and beyond the diffraction limit / Hari Shroff.
Author: Shroff, Hari.
National Institutes of Health (U.S.),
Publisher:
Abstract: (CIT): Director's Seminar Series Dr. Hari Shroff will discuss his lab's, the Section of High Resolution Optical Imaging (HROS), efforts to improve structured illumination microscopy (SIM) and light-sheet microscopy. SIM doubles the spatial resolution of light microscopy, requiring lower light intensities and acquisition times than other super-resolution techniques. Dr. Shroff will present SIM implementations that enable resolution doubling in live samples > 10-20x thicker than possible with conventional SIM, as well as hardware modifications that enable effectively "instant" SIM imaging at rates 10-100x faster than other SIM. New applications of instant SIM, including combination with total internal reflection (TIRF) and with adaptive optics will also be discussed. The second half of the talk will focus on the development of inverted selective plane illumination microscopy (iSPIM), and subsequent application to the noninvasive study of neurodevelopment in nematodes. Dr. Shroff will discuss progress that quadruples the axial resolution of iSPIM by using a second specimen view, enabling imaging with isotropic spatial resolution (dual-view iSPIM, or diSPIM). Newer multiview microscopes with more objectives and more views, further improving spatial resolution, will also be described. Applications of these technologies will be presented, including computational methods for untwisting worm embryos and calcium imaging in freely moving embryos. Finally, Dr. Shroff will discuss the current status of the new trans-NIH, advanced imaging and microscopy (AIM) facility which allows intramural researchers to use cutting edge optical imaging systems developed at NIH.
Subjects: Image Processing, Computer-Assisted
Microscopy
Optical Imaging
Publication Types: Lectures
Webcasts
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NLM Classification: QH 215
NLM ID: 101707000
CIT Live ID: 23395
Permanent link: https://videocast.nih.gov/launch.asp?23298