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Antibodies, Genome Stability, and Cancer

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Air date: Wednesday, March 27, 2013, 3:00:00 PM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 435, (108 Live, 327 On-demand)
Category: WALS - Wednesday Afternoon Lectures
Runtime: 01:10:05
Description: Wednesday Afternoon Lecture Series

Dr. Alt and his group developed high-throughput, genome-wide translocation sequencing (HTGTS) to elucidate mechanisms that generate and join DNA double strand breaks (DSBs) and mediate translocations. He will discuss several new studies that employ the HTGTS approach and other approaches to address questions about the immunoglobulin heavy locus (IgH) class switch recombination (CSR) including how CSR DSBs are joined in a productive (deletional versus inversional) orientation in the chromosome; how IgH DSBs are synapsed for joining over several 100-kilobyte chromosomal distances to effect physiological levels of CSR; and the potential roles of the nuclear protein kinase ataxia telangiectasia mutated (ATM)-dependent DNA DSB response factors, including 53BP1, in the CSR process. He will also discuss the use of HTGTS to elucidate novel mechanisms that lead to V(D)J recombination-associated oncogenic chromosomal translocations and oncogene activation in ATM-deficient B- and T-cell lymphomas.
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NLM Title: Antibodies, genome stability, and cancer / Fred Alt.
Series: Wednesday afternoon lecture series
Author: Alt, Frederick W.
National Institutes of Health (U.S.),
Publisher:
Other Title(s): Wednesday afternoon lecture series
Abstract: (CIT): Wednesday Afternoon Lecture Series Dr. Alt and his group developed high-throughput, genome-wide translocation sequencing (HTGTS) to elucidate mechanisms that generate and join DNA double strand breaks (DSBs) and mediate translocations. He will discuss several new studies that employ the HTGTS approach and other approaches to address questions about the immunoglobulin heavy locus (IgH) class switch recombination (CSR) including how CSR DSBs are joined in a productive (deletional versus inversional) orientation in the chromosome; how IgH DSBs are synapsed for joining over several 100-kilobyte chromosomal distances to effect physiological levels of CSR; and the potential roles of the nuclear protein kinase ataxia telangiectasia mutated (ATM)-dependent DNA DSB response factors, including 53BP1, in the CSR process. He will also discuss the use of HTGTS to elucidate novel mechanisms that lead to V(D)J recombination-associated oncogenic chromosomal translocations and oncogene activation in ATM-deficient B- and T-cell lymphomas.
Subjects: DNA Breaks, Double-Stranded
High-Throughput Nucleotide Sequencing
Immunoglobulin Class Switching
Sequence Analysis, DNA
Translocation, Genetic--immunology
Publication Types: Lectures
Webcasts
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Caption Text: Download Caption File
NLM Classification: QU 477
NLM ID: 101607218
CIT Live ID: 12314
Permanent link: https://videocast.nih.gov/launch.asp?17874