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Evidence that Yeast DNA Polymerase Participates in Leading Strand Replication

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Air date: Tuesday, March 20, 2007, 12:30:00 PM
Time displayed is Eastern Time, Washington DC Local
Views: Total views: 104 * This only includes stats from October 2011 and forward.
Category: DNA Repair
Runtime: 00:18:01
Description: Multiple DNA polymerases participate in replicating the leading and lagging strands of the eukaryotic nuclear genome. Although 50 years have passed since the first DNA polymerase was discovered, the identity of the major polymerase used for leading-strand replication is uncertain. We constructed a derivative of yeast DNA polymerase e that retains high replication activity but has strongly reduced replication fidelity, particularly for thymine-deoxythymidine 5’-monophosphate (T-dTMP) but not adenine-deoxyadenosine 5’-monophosphate (A-dAMP) mismatches. Yeast strains with this DNA polymerase e allele have elevated rates of T to A substitution mutations. The position and rate of these substitutions depend on the orientation of the mutational reporter and its location relative to origins of DNA replication and reveal a pattern indicating that DNA polymerase e participates in leading-strand DNA replication.

The DNA Repair Interest Group is concerned with all forms of DNA damage and repair. As a major defense against environmental damage to cells DNA repair is present in all organisms examined including bacteria, yeast, drosophila, fish, amphibians, rodents and humans. The members of the DNA Repair Interest Group perform research in areas including DNA repair enzymology and fine structure, mutagenesis, gene and cell cycle regulation, protein structure, and human disease.

For more information, visit the
DNA Repair Interest Group
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Author: Zachary Pursell, Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH
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CIT Live ID: 6049
Permanent link: https://videocast.nih.gov/launch.asp?13941