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Multiple DNA polymerases participate in replicating the leading and lagging strands of the
eukaryotic nuclear genome. Although 50 years have passed since the first DNA polymerase was
discovered, the identity of the major polymerase used for leading-strand replication is uncertain.
We constructed a derivative of yeast DNA polymerase e that retains high replication activity but has
strongly reduced replication fidelity, particularly for thymine-deoxythymidine 5-monophosphate
(T-dTMP) but not adenine-deoxyadenosine 5-monophosphate (A-dAMP) mismatches. Yeast strains
with this DNA polymerase e allele have elevated rates of T to A substitution mutations. The position
and rate of these substitutions depend on the orientation of the mutational reporter and its
location relative to origins of DNA replication and reveal a pattern indicating that DNA polymerase
e participates in leading-strand DNA replication.
The DNA Repair Interest Group is concerned with all forms of DNA damage and repair. As a major defense against environmental damage to cells DNA repair is present in all organisms examined including bacteria, yeast, drosophila, fish, amphibians, rodents and humans. The members of the DNA Repair Interest Group perform research in areas including DNA repair enzymology and fine structure, mutagenesis, gene and cell cycle regulation, protein structure, and human disease.