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Imaging Synaptic Plasticity in Vivo
Monday, February 23, 2004,
12:00:00 PM Time displayed is Eastern Time, Washington DC Local
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Karel Svoboda’s lab studies the electrical and biochemical dynamics of the neocortex and how these dynamics change in response to experience. To approach these questions, the lab studies how the basic units of the network, neurons and their synapses, work within the intact network using sensitive optical tools. Two-photon laser scanning microscopy allows them to detect excitation of single synapses in brain slices and in the intact brain by measuring intracellular calcium accumulation associated with activation of synaptic receptors. Excitation of neuronal elements by focal uncaging of neurotransmitters allows them to probe the connectivity of neural networks.