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A fundamental goal of cell biology is to define the functions of proteins in the context of compartments that organize them in the cellular milieu. We have constructed and analyzed a collection of yeast strains expressing full-length, chromosomally-tagged green fluorescent protein (GFP) fusion proteins. We classified these proteins, representing 75% of the yeast proteome, into twenty-two distinct subcellular localization categories and provide localization information for 70% of previously unlocalized proteins. Analysis of this high-resolution, high-coverage localization dataset in the context of transcriptional and protein-protein interaction data helps reveal the logic of transcriptional co-regulation and provides a comprehensive view of interactions within and between organelles in eukaryotic cells.
High-throughput analysis of subcellular localization in budding yeast [electronic resource] / Erin O'Shea.
Series:
Global analysis of protein localization in budding yeast
Author:
O'Shea, Erin. National Institutes of Health (U.S.)
Publisher:
[Bethesda, Md. : National Institutes of Health, 2004]
Other Title(s):
Global analysis of protein localization in budding yeast
Abstract:
(CIT): A fundamental goal of cell biology is to define the functions of proteins in the context of compartments that organize them in the cellular milieu. We have constructed and analyzed a collection of yeast strains expressing full-length, chromosomally-tagged green fluorescent protein (GFP) fusion proteins. We classified these proteins, representing 75% of the yeast proteome, into twenty-two distinct subcellular localization categories and provide localization information for 70% of previously unlocalized proteins. Analysis of this high-resolution, high-coverage localization dataset in the context of transcriptional and protein-protein interaction data helps reveal the logic of transcriptional co-regulation and provides a comprehensive view of interactions within and between organelles in eukaryotic cells. For more information, visit the Proteomics Interest Group.
Subjects:
Gene Expression Regulation Protein Transport Proteomics Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae
Publication Types:
Government Publications Lectures Webcasts
Rights:
This is a work of the United States Government. No copyright exists on this material. It may be disseminated freely.
